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Principle: Using loop-mediated isothermal amplification technology (LAMP), specific primers are designed for the nucleocapsid protein N gene and ORF1 ab gene of the new coronavirus (2019-nCoV). The reaction system contains the new coronavirus (2019-nCoV).) For nucleic acids, the amplification of exponential amplification and combined with the fluorescent signal, real-time detection of the fluorescence signal during the amplification process using a fluorescence PCR instrument, to achieve the qualitative analysis of the novel coronavirus nucleic acid in the sample.
Detection time: 30 minutes
Test samples: whole blood, plasma, serum, throat swabs
Advantages: short detection time, high sensitivity and strong specificity Disadvantages: low-temperature transportation, high requirements for experimental conditions. reagent composition: buer, DNA polymerase, reverse transcriptase, sealant, positive control, negative control. Minimum detection volume: 1000 copies / mL
Specificity: with human coronavirus HCoV-OC43, human coronavirus HCoV-HKU1, human coronavirus HCoV-229E , human coronavirus HCoV-NL63 , adenovirus, respiratory syncytial virus type A, human parainfluenza type 2 virus, human parainfluenza 3 virus, H1N1 subtype of influenza virus, the H5N1 subtype of influenza A virus, H7N9 subtype of influenza A virus, H9N2 subtype of influenza virus, Mycoplasma pneumonia, influenza B viruses are nothing more than a specific application. Precision: Two cases of high- and low-concentration positive control were repeated 10 times, and their Ct value was CV≤5%.