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Catalog # PLCP12Product Storage 4 ºCPoly-L-lysine Coated plate are manufactured to support and promote cell growth.Collagen Coated plate achieve optimal differentiation. Enhancement of neuronal cellattachment to plastic and glass, promotion of neurite outgrowth, culture of glial cells asa feeder layer for neurons, construction of neural cell model systems to study CNSfunction, development and diseases.The lower molecular weight poly-L-lysine (30-70 kDa) is easier to use because it is lessviscous in solution, but the higher molecular weight poly-L-lysine (>300 kDa) providesmore attachment sites per molecule. The mid-range product has a MW range of 70-150kDa. There might be a small amount of product in the β structure, but very little. This isbecause the HBr interferes with hydrogen bonding between the amino and carboxylgroups, or between the amino group and other N or O containing moieties. Thesecondary structures of polyamino acids have never been extensively studied, but it isknown that the degree of secondary conformational regularity depends on the aminoacid in the polymer. Plates can be used in a broad range of applications, including theculturing of fresh or frozen hepatocytes (rat and human), as well as for neuronal,epithelial, and muscle primary cells. They are also ideal for culturing human cornealepithelial cells (HCEC) in defined media.
The coated plate bound to 6, 12, 24 and 96 well plates, you can routinely venturebeyond the rigidity of tissue culture plastic. STABILITY Stable for a minimum of 3months from date of shipment when stored at 4ºCRecommended Plating Volumes of Cell Culture Plate Growth Area (cm2) Volume PerWell (µl)Coated plate Growth Area (cm2) Volume Per Well (µl)6-well 9.60 120024-well 2.00 25096-well 0.32 50