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Product Name: Neonatal Rat Cardiac FibroblastsCatalog No: R1015Product Format: Frozen or ProliferatingCell Number: >90% confluent in T-25 flask/Frozen vialStorage: 37°C or liquid NitrogenGeneral Information: Neonatal Rat Cardiac Fibroblasts cells were isolated fromnormal neonatal SD rats heart tissue. Passage 1 Neonatal Rat Cardiac Fibroblastscell can be shipped proliferated in a T-25 flask at >90 confluence, or frozen vial (> 5x 105 cells). DMEM contains 5% Fetal calf serum (Full medium) is recommended forcell culture and these cells have an average minimum population doubling levels >8when cultured following the detailed protocol. Cells are tested negative for commonexperimental animal pathogens, and mycoplasma in vitro. When you receive thecells, leave the flask in 37°C CO2 incubator for 1 hour first, and then replace thetransport medium with fresh Full medium. Let the cells grow for 24hours beforesubculture.Subculturing Proliferated Cells:1. Rinse the cells in T25 flask with 5ml DPBS (Room Temperature, RT) twice.2. Add 2ml of Trypsin/EDTA (RT) into T25flask (make sure the whole surface ofthe T25 flask is covered with Trypsin/EDTA),and gently dispose theTrypsin/EDTA solution within 10 seconds with aspiration.3. Leave the T25 flask with the cells at RT for 1 minute (the cells will normallycome off the surface within 1-2 minute). Suspend the cells with 40ml of freshFull medium and the cell suspension is transferred directly into 4 x T25 flasks(10ml each, and the cells are subcultured at 1:4ratio).4. Culture medium (full medium) is changed every 2-3 days. The cells normallybecome confluent within 7 days (when split with a ratio of 1:4).
Product Information:Tissue Origin: SD rats heart tissue.Passage: 1Storage Conditions: Liquid Nitrogen− Vapor Phase OnlySpecial Handling: Minimize exposure to non−liquid nitrogen temperatures, especially roomTemperature transfers.Date of Expiration: Guaranteed for up to 6 months from date of shipment when stored inLiquid Nitrogen.Required Testing and ResultsTest Specification ResultsCell Count > 1x105viable cells/vial ~1.90×105 Cells/VialCell Viability >90% 98%Endotoxin <3 EU/mL <1 EU/mLUSP Sterility
Negative NegativeMycoplasma Negative NegativeAppearance, ContainerIntegrityConforms ConformsAppearance, SuspensionAppearanceConforms ConformsProduct Handling:Caution: The user should treat all cells as potential pathogens. Wear protective clothing andeye wear. Practice appropriate disposal techniques for potentially pathogenic orbiohazardous materials.For Research Use Only. Not for use in diagnostic procedures. If you have any furtherquestions about this Certificate of Analysis, please contact us