Product Name: MEM Alpha Modified w/ L-Glutamine, Ribo, DeoxyribonucleosidesCatalog No: PG066Product Format: LiquidStorage: 4°C
General Information: Minimum Essential Medium (MEM), developed by Harry Eagle, is oneof the most widely used of all synthetic cell culture media. Early attempts to cultivatenormal mammalian fibroblasts and certain subtypes of HeLa cells revealed that they hadspecific nutritional requirements that could not be met by Eagle’s Basal Medium (BME).Subsequent studies using these and other cells in culture indicated that additions to BMEcould be made to aid growth of a wider variety of fastidious cells. MEM, whichincorporates these modifications, includes higher concentrations of amino acids so that themedium more closely approximates the protein composition of mammalian cells. MEM hasbeen used for cultivation of a wide variety of cells grown in mono-layers.The medium is for research use only and not approved for human or animal use or forapplication in any in vitro diagnostic procedure. Storage of the medium and supplementsPlease note that the basal medium has to be stored at 4°C up to 8°C but never should befrozen. The supplement has to be stored at -20°C. Protect from light for a longer timeperiod.Preparing and Stability:Thaw the supplement mixture at 37°C before adding to the basal medium. After thawingplease rinse the bottle and the tube with 70% ethanol as protection. Remove ethanolexcess before opening the cap of the tube. After adding the supplement mixture to thebasal medium slew carefully to get a homogenous medium. Please be careful not totouch the tube or bottle interior threads with your fingers. Please avoid long termexposure to light because some components are light sensitive. Also do not warm up themedium over 37°C. All this protects your medium against contamination or degradationof essential components.