Matrix Hydrogel Coated Plate

Catalog # MHCP12
Product Storage 4 ºC
Extracellular matrix (ECM) proteins play an important role in the regulation of
fundamental cellular processes and are widely used as a coating or matrix for tissue
culture systems to permit the development of models that closely mimic in
vivo conditions
Matrix Hydrogels Coated plate are manufactured using collagen product and
formulated with other ECM to support and promote cell growth. Matrix Hydrogel Coated
plate is a synthetic matrix that is used to achieve optimal differentiation. Matrix
Hydrogels coated plate bound to 6, 12, 24 and 96 well plates targeting 9 levels of
stiffness (kPa).
Soft: 0.2, 0.5, 1
Mid: 2, 4, 8
Stiff: 12, 25, 50

The product regulated stiffness replicates a broad range of physiological tissue
softness, from fat to cardiac muscle, so you can routinely venture beyond the rigidity of
tissue culture plastic.
Our Matrix Hydrogel Coated Plate help with but not limited to:
Embryonic Cell:
The expression of stem cells markers.
Hepatocytes:
Help with myofibroblastic differentiation in Hepatic stellate cells.
Neural:
Promote neuro branching of SH-SY5Y cell line, and other Human Neuro Cells.
Heart:
Help gene expression and phenotype in neonatal cardiomyocytes in vitro.
Bone, Cartilage Skeletal Muscle:
Increases cellular communication, and regulates skeletal muscle stem cell self-renewal
in culture.
General and Mechanistic:
Influence of substrate stiffness on circulating progenitor cell fate. Integrin activation and
internalization on soft ECM as a mechanism of induction of stem cell differentiation by
ECM elasticity
Eye:
Substrate elasticity as biomechanical modulator of tissue homeostatic parameters in
corneal keratinocytes.
Endothelia Cell:
Endothelial barrier disruption and recovery is controlled by substrate stiffness. The
combined influence of substrate elasticity and surface-grafted molecules on the ex vivo
expansion of hematopoietic stem and progenitor cells

Mesenchymal:
Effect of matrix stiffness on the differentiation of mesenchymal stem cells in response to
TGF-β.
STABILITY Stable for a minimum of 3 months from date of shipment when stored at 4ºC
Recommended Plating Volumes of Cell Culture Plate Growth Area (cm2) Volume Per
Well (µl)
Hydrogels Coated plate Growth Area (cm2) Volume Per Well (µl)
6-well 9.60 1200
24-well 2.00 250
96-well 0.32 50