International Shipping may vary. Get in contact
Home
About Us
Blog
Contact Us
(617) 882-2185
sales@ubpone.com
My Account
Welcome to UBPONE!
Login
Create Account
0
Human Cells
Animal Cells
Neurons
Serum
Others
0
Shopping Cart
Your cart is currently empty.
Continue Shopping
My Account
Welcome to UBPONE!
Login
Create Account
Home
Human Monocytic THP-1 Cells
Human Monocytic THP-1 Cells
Product Description
Name of Products
Human Monocytic THP-1 Cells
Catalogue Number
UBP-0055
Product Format
Frozen Vial
Cell Number
> 5×10[5] cells/vial
GENERAL INFORMATIONTHP-1 cells are isolated and established from human monocytic leukemia of a 1-year old infant. The cells are shipped in frozen vials with > 5 x 105 cells/vial. GFP-THP-1 cells are with THP-1 cultured medium (UBP-43).
CHARACTERIZATION OF THE CELLS
1.
THP-1 cells are tested negative for HIV-1, HBV, HCV, and mycoplasma.
PRODUCT USAGE The cells are offered for Research Use Only.
SHIPPING Frozen Vials in a Dry Ice Package.
HANDLING OF ARRIVING CELLS When you receive the dry ice package with cells in frozen vials, transfer the frozen vials of cells into a -80C freezer for short period storage or a liquid nitrogen tank for long- term storage.
PROTOCOLS FOR THAWING THE CELLS AND SUBCULTURE
A)
Pre-coating of T25 flasks- Add 2ml each Universal Coating Solution (UBP-01) into a T25 flask to cover the whole surface of the flask, 5 mins later, dispose the excessive coating solution by aspiration and the flask is ready to be used.
B)
Thaw the frozen cell vial in a 37C water bath first, and then transfer the cells into the pre-coated T25 flask with 10ml of UBP-43 medium, cells usually become confluent with 5-7 days.
C)
To passage the cells, rinse the cells in a T25 flask with 5ml HBSS (RT) twice; then add 2ml Universal Detachment Solution (RT) (UBP-23) into one T25 flask; gently dispose the excessive Universal Detachment Solution within 20 seconds by aspiration.
D)
Leave the T25 flask with the cells at RT or 37C for 1 min (most cells usually will detach from the surface within 1-2 mins; or monitor the cells under a microscope until most of cells become rounded up, and then gently tap the flask against the bench surface, and the cells will move on the surface of the flask when monitoring under microscope.
E)
Add 5ml Universal Neutralization Buffer and spin down the cells with 800g centrifugation for 5 mins.
G)
Re-suspend the cell pellet with 10 or 15ml Full medium and transfer 5 ml each into 2 or 3 pre-coated T25 flasks (for 1/2 to 1/3 subculture ratio).
H)
Change medium every 2 or 3days and the cells usually become confluent within 7 days (when split at a 1/3 ratio).
Default Title - $760.00 USD
$760.00
This item is a recurring or deferred purchase. By continuing, I agree to the
cancellation policy
and authorize you to charge my payment method at the prices, frequency and dates listed on this page until my order is fulfilled or I cancel, if permitted.
Maximum quantity available reached.
Related products