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Name of Cells:
Product Format: Cell Number:
Human Liver Stellate Clls (CI-HLiStCs)
CI-HLiStCs (UBP-0071) are selected from HLiStCs (UBP-0035) infected with lentiviruses expressing SV40 LT antigen under the control of Tet-On system resistant puromycin. CI-HLiStCs are maintained with proliferative capacity in the presence of DoxycyclineNOTE1 in Stellate-Growth medium (contains 10% serum and growth supplements, Cat# UBP-31).
CI-HLiStCs are negative for HIV-1, HBV, HCV, and mycoplasma.
Product Use: CI-HLiStCs are for research use onlyNOTE3.
Shipping: Frozen Vial.
When you receive the cells in a frozen vial, you can transfer the vial of cells into a -80ºC freezer for short period storage or a liquid nitrogen tank for long term storage. Thaw the cells in a 37°CNOTE2 water bath, and then transfer the cells into a T25 flask pre-coated with Universal Coating Solution (UBP01) as described in detail in Subculture Protocol.
NOTE 1: To minimize the effect of SV40 antigen LT for your studies, cells could be cultured in the absence Doxycycline for 3-5 days.
NOTE 2: Although wild type SV40 LT antigen is used for cell immortalization, we noticed that the immortalized cells are growing better at 33-34ºC, we encourage the end users to switch to 33-34ºC if cells are growing slower at 37ºC.
NOTE 3: The purchase of this product conveys to the buyer the nontransferable right to use the purchased amount of the product and all replicates and derivatives for research purposes conducted by the buyer in his/her laboratory only (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes.