Catalog # HBHCP12Product Storage 4 ºCHuman Base Hydrogels Coated plate are manufactured using collagen productIsolated from Bovine Tendon, the product is formulated with other ECM to support andpromote cell growth. Human Base Hydrogels coated plate bound to 6, 12, 24 and 96well plates targeting 9 levels of stiffness (kPa).Soft: 0.2, 0.5, 1Mid: 2, 4, 8Stiff: 12, 25, 50The product regulated stiffness replicates a broad range of physiological tissuesoftness, from fat to cardiac muscle, so you can routinely venture beyond the rigidity oftissue culture plastic.
Our Human Base Hydrogel Coated Plate help with but not limited to:Embryonic Cell:The expression of stem cells markers.Hepatocytes:Help with myofibroblastic differentiation in Hepatic stellate cells.Neural:Promote neuro branching of SH-SY5Y cell line, and other Human Neuro Cells.Heart:Help gene expression and phenotype in neonatal cardiomyocytes in vitro.Bone, Cartilage Skeletal Muscle:Increases cellular communication, and regulates skeletal muscle stem cell self-renewalin culture.General and Mechanistic:Influence of substrate stiffness on circulating progenitor cell fate. Integrin activation andinternalization on soft ECM as a mechanism of induction of stem cell differentiation byECM elasticityEye:Substrate elasticity as biomechanical modulator of tissue homeostatic parameters incorneal keratinocytes.Endothelia Cell:Endothelial barrier disruption and recovery is controlled by substrate stiffness. Thecombined influence of substrate elasticity and surface-grafted molecules on the ex vivoexpansion of hematopoietic stem and progenitor cells.Mesenchymal:Effect of matrix stiffness on the differentiation of mesenchymal stem cells in response toTGF-β.STABILITY Stable for a minimum of 3 months from date of shipment when stored at 4ºC
Recommended Plating Volumes of Cell Culture Plate Growth Area (cm2) Volume PerWell (µl)Human Base HydrogelsCoated plateGrowth Area (cm2) Volume Per Well (µl)6-well 9.60 120024-well 2.00 25096-well 0.32 50