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HLFCs-Ad are isolated from healthy human lung tissue (adult) samples and shipped in frozen vials (the cells are provided @ passage 1). DMEM contains 10% Fetal Bovine Serum (Full medium) is recommended for cell culture and these cells have an average minimum population doubling levels > at least 14 when cultured following the detailed protocol described below). HLFCs-Ad are tested negative for HIV-1, HBV, HCV, and mycoplasma.
Product Use: HLFCs-Ad are for research use only.
Shipping: Frozen Vial.
Handling of Arriving Cells
When you receive the cells in a frozen vial, you can transfer the vial of cells into a -80ºC freezer for short term storage or a liquid nitrogen tank for long term storage. Thaw the cells in a 37°C water bath, and then transfer the cells into a T25 flask pre-coated with Universal Coating Solution (UBP-01) as described in detail in Subculture Protocol.
Change medium every 2-3 days and cells usually become confluent within 7 days (when split at a 1:3 ratio).