ENDOTHELIAL BASAL MEDIUM

Description

Product Name ENDO- Basal Medium
Catalog Number EDBM-2101
Size 500 ml
Storage 2-8°C

Caution: Proper precautions must be taken to avoid exposure. Always wear proper protective equipment
(Gloves, safety glasses, etc.) when handling these materials. We recommend following the universal
procedures for handling products of human origin as the minimum precaution against contamination.
The listed dilutions are for recommendation only and the ender users should optimize the final conditions.
Product Information
Product Name: Endothelial basal medium
Catalog No: EDBM-2101
Size: 500ml
Storage: 2-8°C
Description
The Endothelial basal medium is a chemically defined medium (details of the Formulation
for the medium is enclosed in this data sheet) that was designed to culture endothelial cells
under low serum condition with the support of other growth factors.
Shipping Condition: Ambient temperature (blue ice, seasonally).
Storage Condition:
The Endothelial basal medium (EDBM) is stored at 2-8°C. A change in color or appearance
of precipitate may indicate deterioration.
Shelf Life: 3 months from the date of receipt under proper storage condition.
Use of ENDO-Basal Medium
Use of EDBM to Prepare Full Endothelial Growth Medium (EGM-2102) for Culturing
Endothelial Cells:
1) Thaw Endothelial Growth supplement (EGS-1001) and add 25 ml (the whole package
size) of ENDO Growth supplement into 475ml of ENDO-Basal medium (total volume 500ml,
with 5% fetal bovine serum and recombinant growth factors). Antibiotics are already
provided in ENDO-Growth supplement, which makes the final concentration of antibiotics
as 1 x penicillin and streptomycin.

2) Take the amount of ENDO-Growth medium needed and warm to 37°C in a water bath or
incubator.
3) Avoid frequent temperature change to the entire bottle of medium.
4) The ENDO-growth medium is stable for at least 3 month stored at 2-8°C. We recommend
changing medium every 2 days for regular cell culture