Product Name: Eagle’s Basal Medium (BME)Catalog No: PG014Product Format: LiquidStored at 4°C (BME)
_____________________________________________________________________General Information: Eagle’s Basal Medium (BME) was developed by Harry Eagle. BMEforrmula was developed as a result of numerous studies carried out in the late 1950’s todetermine the essential nutritional requirements and other factors critical to the growth ofcells in culture. Historically, BME has been used in studies conducted to measure thegrowth response of normall (WI-38) and transformed (mouse and HeLa) cells in monolayergrowth. It is one of the most widely used of all synthetic cell culture media.The medium is for research use only and not approved for human or animal use or forapplication in any in vitro diagnostic procedure. Storage of the medium andsupplements Please note that the basal medium has to be stored at 4°C up to 8°C butnever should be frozen. The supplement has to be stored at -20°C. Protect from light fora longer time period.Preparing and Stability:Thaw the supplement mixture at 37°C before adding to the basal medium. After thawingplease rinse the bottle and the tube with 70% ethanol as protection. Remove ethanolexcess before opening the cap of the tube. After adding the supplement mixture to thebasal medium slew carefully to get a homogenous medium. Please be careful not to touchthe tube or bottle interior threads with your fingers. Please avoid long term exposure tolight because some components are light sensitive. Also do not warm up the medium over37°C. All this protects your medium against contamination or degradation of essentialcomponents.