Product Name: Dulbecco’s MEM (DMEM) w/ HEPES w/o L-Glutamine
Catalog No: PG044
Product Format: Liquid
Stored at 4°C
General Information: Dulbecco’s MEM (DMEM) w/ HEPES w/o L-Glutamine is the most
widely used modification of BME. It contains a 4-fold higher concentration of amino acids
and vitamins. Non-essential amino acids and certain essential trace elements were added.
The bicarbonate concentration was increased. The standard formula for DMEM is with
1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic
cells. Today, it finds a broad application of serum free culture of normal and transformed
mouse and chicken cells.
The medium is for research use only and not approved for human or animal use or for
application in any in vitro diagnostic procedure. Storage of the medium and
supplements Please note that the basal medium has to be stored at 4°C up to 8°C but
never should be frozen. The supplement has to be stored at -20°C. Protect from light for
a longer time period.
Preparing and Stability:
Thaw the supplement mixture at 37°C before adding to the basal medium. After thawing
please rinse the bottle and the tube with 70% ethanol as protection. Remove ethanol
excess before opening the cap of the tube. After adding the supplement mixture to the
basal medium slew carefully to get a homogenous medium. Please be careful not to touch
the tube or bottle interior threads with your fingers. Please avoid long term exposure to
light because some components are light sensitive. Also do not warm up the medium over
37°C. All this protects your medium against contamination or degradation of essential