Product Name: Dulbecco’s MEM (DMEM) w/ HEPES w/o L-GlutamineCatalog No: PG044Product Format: LiquidStored at 4°C
General Information: Dulbecco’s MEM (DMEM) w/ HEPES w/o L-Glutamine is the mostwidely used modification of BME. It contains a 4-fold higher concentration of amino acidsand vitamins. Non-essential amino acids and certain essential trace elements were added.The bicarbonate concentration was increased. The standard formula for DMEM is with1000mg/ml glucose. DMEM was originally developed for the culture of mice embryoniccells. Today, it finds a broad application of serum free culture of normal and transformedmouse and chicken cells.The medium is for research use only and not approved for human or animal use or forapplication in any in vitro diagnostic procedure. Storage of the medium andsupplements Please note that the basal medium has to be stored at 4°C up to 8°C butnever should be frozen. The supplement has to be stored at -20°C. Protect from light fora longer time period.Preparing and Stability:Thaw the supplement mixture at 37°C before adding to the basal medium. After thawingplease rinse the bottle and the tube with 70% ethanol as protection. Remove ethanolexcess before opening the cap of the tube. After adding the supplement mixture to thebasal medium slew carefully to get a homogenous medium. Please be careful not to touchthe tube or bottle interior threads with your fingers. Please avoid long term exposure tolight because some components are light sensitive. Also do not warm up the medium over37°C. All this protects your medium against contamination or degradation of essentialcomponents.