Dulbecco’s MEM (DMEM) Ham’s F-12

Description

Product Name: Dulbecco’s MEM (DMEM) Ham’s F-12
Catalog No: PG029
Product Format: Liquid
Stored at 4°C

General Information: Dulbecco’s MEM (DMEM) Ham’s F-12 w/o Arginine, Leucine, Lysine
is the most widely used modification of BME. It contains a 4-fold higher concentration of
amino acids and vitamins. Non-essential amino acids and certain essential trace elements
were added. The bicarbonate concentration was increased. The standard formula for
DMEM is with 1000mg/ml glucose. DMEM “High Glucose“ with 4500mg/ml aides in the
cultivation of certain cell types. DMEM was originally developed for the culture of mice
embryonic cells. Today, it finds a broad application of serum free culture of normal and
transformed mouse and chicken cells. Ham’s Nutrient Mixtures were originally developed
to support growth of several clones of Chinese hamster ovary (CHO) cells, of HeLa and of
mouse L-cells. Ham’s mixtures were formulated for use with or without serum
supplementation, depending on the cell type being cultured. Ham’s F-12 was developed for
growth of primary rat hepatocytes and rat prostate epithelial cells. It is also used in a
clonal toxicity assay using CHO cells. The medium is for research use only and not
approved for human or animal use or for application in any in vitro diagnostic
procedure. Storage of the medium and supplements Please note that the basal medium
has to be stored at 4°C up to 8°C but never should be frozen. The supplement has to be
stored at -20°C. Protect from light for a longer time period.
Preparing and Stability:
Thaw the supplement mixture at 37°C before adding to the basal medium. After thawing
please rinse the bottle and the tube with 70% ethanol as protection. Remove ethanol
excess before opening the cap of the tube. After adding the supplement mixture to the
basal medium slew carefully to get a homogenous medium. Please be careful not to touch
the tube or bottle interior threads with your fingers. Please avoid long term exposure to
light because some components are light sensitive. Also do not warm up the medium over
37°C. All this protects your medium against contamination or degradation of essential
components.