CI-HNCs (UBP-0070) are selected from HNCs (UBP-0036) infected with lentiviruses expressing SV40 LT antigen under the control of Tet-On system resistant puromycin. CI-HNCs are maintained with proliferative capacity in the presence of Doxycycline in HNCs Growth Medium (contains 10% serum and growth supplements, UBP-34).
The cells are positive for several neuronal markers:
Neuron specific enolase (NSE)
The cells are negative for
Glial fibrillary acidic protein (GFAP)
Myelin basis protein (MBP)
HNCs are negative for HIV-1, HBV, HCV, and mycoplasma.
Product Use: CI-HNCs are for research use onlyNOTE3
Shipping: Frozen Vials in Dry Ice packages
Handling of Arriving Cells:
When you receive the cells in a frozen vial, you can transfer the vial of cells into a -80ºC freezer for short-period storage or a liquid nitrogen tank for long-term storage. Thaw the cells in a 37°C water bath, and then quickly transfer the cells into a T75 flask with 15 ml MSCGM in the presence of 1ug/ml of Doxycycline and incubated overnight in a 37ºC, CO2 incubator and change the medium next day (15 ml complete MSCGM) and every other thereafter.