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Principle: The principle of immunocapture method is used to detect the new coronavirus IgM antibody and IgG antibody. The nitrocellulose membrane was coated with a mouse anti-human IgM (μ chain) monoclonal antibody, a mouse anti-human IgG (γ chain) monoclonal antibody, and a goat anti-mouse IgG antibody, respectively. Colloidal gold-labeled recombinant new coronavirus (nCoV) antigen and A murine IgG antibody was used as a tracer. When using, add the sample (sample) to the sample wells of the detection reagent card for detecting IgM antibody and IgG antibody. If the sample contains nCoVIgM antibody, it can be combined with colloidal gold-labeled nCoV antigen to form a complex. The coated mouse anti-human IgM antibody is captured and combined with the mouse anti-human IgM antibody to form a complex with a purple-red band; if the sample contains the nCoVIgG antibody, it can be combined with colloidal gold-labeled nCoV antigen to form a complex The complex binds to a mouse anti-human IgG ( γ chain) monoclonal antibody to form a complex with a purple-red band. The colloidal gold-labeled murine IgG antibody showed a purplish red band on the coated goat anti-mouse IgG antibody as a quality control line.
Detection time: 10-15 minutes
Test samples: whole blood, plasma, serum
Advantages: transportation at room temperature, fast detection time, low laboratory requirements, suitable for large-scale rough screening
Disadvantages: prone to false positives
Colloidal Gold Rapid Test
Reagent composition: test card, sample diluent, desiccant.
Special samples to avoid: Hyperlipidemia serum (triglyceride concentration greater than 25.3mg / ml), bilirubin serum (concentration greater than 0.2mg / ml) , hemolytic serum (hemoglobin concentration greater than 5.0mg / ml ). Red background and other phenomena may appear, which have certain influence on the judgment of inspection results and should be avoided. influenza, A type IgM antibody, Incense virus B type IgM antibody, parainfluenza virus IgM antibody, Mycoplasma pneumoniae IgM antibody, Chlamydia pneumoniae IgM antibody, Respiratory syncytial virus IgM antibody, Adenovirus IgM antibody, Coxsackie virus group B IgM antibody No cross reaction with this product.
Incense virus A type IgG antibodies, incense virus B type IgG antibodies, paranoia virus IgG antibodies, antibody Mycoplasma pneumoniae, Chlamydia pneumoniae IgG , respiratory syncytial virus IgG antibodies, adenovirus IgG antibodies and coxsackie B group IgM antibody of the present No cross-reaction Rheumatoid factor ( RF ), antinuclear antibody ( ANA ), antimitochondrial antibody ( AMA ) have no cross-reaction with this product;