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Precautions and Disclaimer
The handling of any organism derived products has the potential to be biologically hazardous. Proper precautions must be taken to avoid
exposure. Always wear proper protective equipment (Gloves, safety glasses, etc.) when handling these materials. We recommend following the
universal procedures for handling products of any organism’s origin as the minimum precaution against contamination. All product offered by
Universal BioProducts. are for laboratory research purposes only. Any other use and results of that use are the sole responsibility of the user and are
not in any way the responsibility of Universal BioProducts..
Product Data and Description
Collagel Hydrogel Soft-Bovine (ACGH-003) is a biocompatible complex of type I collagen fibers that will
help accelerate the pace of your biomedical and cell/3D tissue engineering applications. Collagel
Hydrogel Soft-Bovine (ACGH-003) contains our high quality, sterile type I tendon collagen which has
been specially formulated for ease of gel formation. Once in a 3D tissue model, the ACGH-003 will not
break or tear apart easily when stretched. ACGH-003 can also be flowable, allowing it to be readily used
as an injectable, biocompatible drug delivery matrix on animal models. To control release of your drug it
is necessary to bind your active agent to ACGH-003 by covalent or non-covalent bonds, or by
sequestering in a secondary matrix. Other potential applications of our CGHH’s are as orthopedic
adhesives via their swelling ability, as scaffolds for bone infiltration and formation through their mesh
structure, as isolators to retain cells, or as gene delivery complexes. The high quality and convenience of
our ACGH-003 products will improve the performance of your biomedical and cell/3D tissue culture
applications. ACGH-003 is an ideal matrix for growing fibroblasts, primary hepatocyte culture and for
growing smooth muscle cells. You will be able to get adipogenesis with MSCs using our Collagel Hydrogel
Soft-Bovine (ACGH-003). For cells/tissue applications that require a less rigid or a more porous matrix,
try our other Collagel Hydrogel Products.
Catalog Number ACGH-003
Cell Culture Testing Pass
Source Bovine Tendon
Shelf Life ≥ 12 months
Storage -20°C
Purity >95% SDS Page
pH 7.0
Conductivity 0.8 mS /cm
Sterility Pass
Endotoxin Level ≤ 1EU/ ml

cautions:
Everything should be kept cold to avoid the Collagel Hydrogel Soft-Bovine (ACGH-003) from solidifying.
Avoid air bubbles. This protocol is based on 6-well plate usage.
Recommended 3 x 10⁶ cells or a fully confluent T75
20 ml Collagel Hydrogel Soft-Bovine DMEM
Method:
Thaw your Collagel Hydrogel Soft-Bovine (ACGH-003) sample bottle at room temperature or in a 37°C
water bath, invert product while thawing. When you see a small amount of ice in your sample, transfer
the bottle into an ice bath. It is important to keep Collagel Hydrogel Soft-Bovine (ACGH-003) on ice since
it will solidify at temperature above 8°C.
1) Place a sterile magnetic stir bar in a sterile beaker
2) Place plate containing ice on a stir plate
3) Place the sterile beaker containing the stir bar on the ice bath
4) Pour the Collagel Hydrogel Soft-Bovine (ACGH-003) into the beaker carefully, try to avoid air
bubbles
5) Slowly start stirring Collagel Hydrogel Soft-Bovine (ACGH-003) .
6) Add your wanted media to the Collagel Hydrogel Soft-Bovine (ACGH-003) solution. Judge the pH
visually by the phenol red in the media then add your cell suspension.
7) Pipette your wanted volume of the mixture into each well.
8) Let 6-well plate sit at room temperature for 10-15minutes before placing in the incubator
9) 1 hour later add your wanted volume of media on top of each Collagel Hydrogel Soft-Bovine
(ACGH-003) (2-2.5 ml)
10) Use within 1 week, change media every 2 days or as needed.

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