Precautions and DisclaimerThe handling of any organism derived products has the potential to be biologically hazardous. Proper precautions must be taken to avoidexposure. Always wear proper protective equipment (Gloves, safety glasses, etc.) when handling these materials. We recommend following theuniversal procedures for handling products of any organism’s origin as the minimum precaution against contamination. All product offered byUniversal BioProducts. are for laboratory research purposes only. Any other use and results of that use are the sole responsibility of the user and arenot in any way the responsibility of Universal BioProducts..Product Data and DescriptionCollagel Hydrogel Soft-Bovine (ACGH-003) is a biocompatible complex of type I collagen fibers that willhelp accelerate the pace of your biomedical and cell/3D tissue engineering applications. CollagelHydrogel Soft-Bovine (ACGH-003) contains our high quality, sterile type I tendon collagen which hasbeen specially formulated for ease of gel formation. Once in a 3D tissue model, the ACGH-003 will notbreak or tear apart easily when stretched. ACGH-003 can also be flowable, allowing it to be readily usedas an injectable, biocompatible drug delivery matrix on animal models. To control release of your drug itis necessary to bind your active agent to ACGH-003 by covalent or non-covalent bonds, or bysequestering in a secondary matrix. Other potential applications of our CGHH’s are as orthopedicadhesives via their swelling ability, as scaffolds for bone infiltration and formation through their meshstructure, as isolators to retain cells, or as gene delivery complexes. The high quality and convenience ofour ACGH-003 products will improve the performance of your biomedical and cell/3D tissue cultureapplications. ACGH-003 is an ideal matrix for growing fibroblasts, primary hepatocyte culture and forgrowing smooth muscle cells. You will be able to get adipogenesis with MSCs using our Collagel HydrogelSoft-Bovine (ACGH-003). For cells/tissue applications that require a less rigid or a more porous matrix,try our other Collagel Hydrogel Products.Catalog Number ACGH-003Cell Culture Testing PassSource Bovine TendonShelf Life ≥ 12 monthsStorage -20°CPurity >95% SDS PagepH 7.0Conductivity 0.8 mS /cmSterility PassEndotoxin Level ≤ 1EU/ ml
cautions:Everything should be kept cold to avoid the Collagel Hydrogel Soft-Bovine (ACGH-003) from solidifying.Avoid air bubbles. This protocol is based on 6-well plate usage.Recommended 3 x 10⁶ cells or a fully confluent T7520 ml Collagel Hydrogel Soft-Bovine DMEMMethod:Thaw your Collagel Hydrogel Soft-Bovine (ACGH-003) sample bottle at room temperature or in a 37°Cwater bath, invert product while thawing. When you see a small amount of ice in your sample, transferthe bottle into an ice bath. It is important to keep Collagel Hydrogel Soft-Bovine (ACGH-003) on ice sinceit will solidify at temperature above 8°C.1) Place a sterile magnetic stir bar in a sterile beaker2) Place plate containing ice on a stir plate3) Place the sterile beaker containing the stir bar on the ice bath4) Pour the Collagel Hydrogel Soft-Bovine (ACGH-003) into the beaker carefully, try to avoid airbubbles5) Slowly start stirring Collagel Hydrogel Soft-Bovine (ACGH-003) .6) Add your wanted media to the Collagel Hydrogel Soft-Bovine (ACGH-003) solution. Judge the pHvisually by the phenol red in the media then add your cell suspension.7) Pipette your wanted volume of the mixture into each well.8) Let 6-well plate sit at room temperature for 10-15minutes before placing in the incubator9) 1 hour later add your wanted volume of media on top of each Collagel Hydrogel Soft-Bovine(ACGH-003) (2-2.5 ml)10) Use within 1 week, change media every 2 days or as needed.