Precautions and DisclaimerThe handling of any organism’s derived products has potential to be biologically hazardous. Proper precautions must be taken to avoidexposure. Always wear proper protective equipment (Gloves, safety glasses, etc.) when handling these materials. We recommend following theuniversal procedures for handling products of any organism’s origin as the minimum precaution against contamination. All product offered byUniversal BioProducts are for laboratory research purposes only. Any other use and results of that use are the sole responsibility of the user and arenot in any way the responsibility of Universal BioProducts.Collagel Hydrogel R (ACGH-001) is a biocompatible complex of type I collagen fibers that will helpaccelerate the pace of your biomedical and cell/3D tissue engineering applications. Collagel Hydrogel R(ACGH-001) contains our high quality, sterile type I tendon collagen which has been specially formulatedfor ease of gel formation. Once in a 3D tissue model, the Collagel Hydrogel R (ACGH-001) will not breakor tear apart easily when stretched. Collagel Hydorgel R (ACGH-001) can also be flowable, allowing it tobe readily used as an injectable, biocompatible drug delivery matrix on animal models. To controlrelease of your drug it is necessary to bind your active agent to Collagel Hydrogel R (ACGH-001) bycovalent or non-covalent bonds, or by sequestering in a secondary matrix. Other potential applicationsof our Collagel Hydrogel R (ACGH-001) are as orthopedic adhesives via their swelling ability, as scaffoldsfor bone infiltration and formation through their mesh structure, as isolators to retain cells, or as genedelivery complexes. The high quality and convenience of our Collagel Hydrogel R (ACGH-001) productswill improve the performance of your biomedical and cell/3D tissue culture applications. CollagelHydrogel R (ACGH-001) is an ideal matrix for growing fibroblasts, primary hepatocyte culture and forgrowing smooth muscle cells. You will be able to get adipogenesis with MSCs using our Collagel HydrogelR (ACGH-001). For cells/tissue applications that require a less rigid or a more porous matrix, try ourother Collagel Hydrogel R (ACGH-001) Produtcs.Catalog Number ACGH-001Cell Culture Testing PassSource Rat TendonShelf Life ≥ 12 monthsStorage -20°CPurity >95% SDS PageConcentration 3 mg/ ml by Sircol AssaypH 7.0Conductivity 0.8 mS/cmSterility PassEndotoxin Level ≤ 1EU/ ml
Precautions:Everything should be kept cold to avoid the collagel Hydrogel R (ACGH-001) from solidifying. Avoid airbubbles. This protocol is based on 6-well plate usage.Recommended 3 x 10⁶ cells or a fully confluent T7520 ml collagel hydrogel R (ACGH-001) DMEMMethod:Thaw your collagel hydrogel R (ACGH-001) sample bottle at room temperature or in a 37°C water bath,invert product while thawing. When you see a small amount of ice in your sample, transfer the bottleinto an ice bath. It is important to keep collagel hydrogel R (ACGH-001) on ice since it will solidify attemperature above 8°C.1) Place a sterile magnetic stir bar in a sterile beaker2) Place plate containing ice on a stir plate3) Place the sterile beaker containing the stir bar on the ice bath4) Pour the collagel hydrogel R (ACGH-001) into the beaker carefully, try to avoid air bubbles5) Slowly start stirring collagel hydrogel R (ACGH-001)6) Add your wanted media to the collagel Hydrogel (ACGH-001) solution. Judge the pH visually bythe phenol red in the media then add your cell suspension.7) Pipette your wanted volume of the mixture into each well.8) Let 6-well plate sit at room temperature for 10-15minutes before placing in the incubator9) 1 hour later add your wanted volume of media on top of each collagel hydrogel R (ACGH-001)(2-2.5 ml)10) Use within 1 week, change media every 2 days or as needed.