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Product Data and Description :
Collagel Hydrogel (ACGH-002) is a biocompatible complex of type Icollagen fibers that will help accelerate the pace of your biomedical and cell/3D tissue engineeringapplications. Collagel Hydrogel (ACGH-002) contains our high quality, sterile type I tendon collagenwhich has been specially formulated for ease of gel formation. Once in a 3D tissue model, the CollagelHydrogel (ACGH-002) will not break or tear apart easily when stretched. Collagel Hydrogel (ACGH-002)can also be flowable, allowing it to be readily used as an injectable, biocompatible drug delivery matrixon animal models. To control release of your drug it is necessary to bind your active agent to CollagelHydrogel (ACGH-002) by covalent or non-covalent bonds, or by sequestering in a secondary matrix.Other potential applications of our Collagel Hydrogel (ACGH-002) are as orthopedic adhesives via theirswelling ability, as scaffolds for bone infiltration and formation through their mesh structure, as isolatorsto retain cells, or as gene delivery complexes. The high quality and convenience of our Collagel Hydrogel(ACGH-002) products will improve the performance of your biomedical and cell/3D tissue cultureapplications. Collagel Hydrogel (ACGH-002) is an ideal matrix for growing fibroblasts, primary hepatocyteculture and for growing smooth muscle cells. You will be able to get adipogenesis with MSCs using ourCollagel Hydrogel (ACGH-002). For cells/tissue applications that require a less rigid or a more porousmatrix, try our other Collagel Hydrogel (ACGH-002) Produtcs.Catalog Number ACGH-002Cell Culture Testing PassSource Rat TendonShelf Life ≥ 12 monthsStorage -20°CPurity >95% SDS PagepH 7.0Conductivity 0.8 mS/cmSterility PassEndotoxin Level ≤ 1EU/ ml
Precautions:Everything should be kept cold to avoid the Collagel Hydrogel (ACGH-002) from solidifying. Avoid airbubbles. This protocol is based on 6-well plate usage.Recommended 3 x 10⁶ cells or a fully confluent T7520 ml Collagel Hydrogel (ACGH-002) DMEMMethods: Thaw your Collagel Hydrogel (ACGH-002) sample bottle at room temperature or in a 37°Cwater bath, invert product while thawing. When you see a small amount of ice in your sample, transferthe bottle into an ice bath. It is important to keep Collagel Hydrogel (ACGH-002) on ice since it willsolidify at temperature above 8°C.1) Place a sterile magnetic stir bar in a sterile beaker2) Place plate containing ice on a stir plate3) Place the sterile beaker containing the stir bar on the ice bath4) Pour the Collagel Hydrogel (ACGH-002) into the beaker carefully, try to avoid air bubbles5) Slowly start stirring collagel Hydrogel (ACGH-002)6) Add your wanted media to the Collagel Hydrogel (ACGH-002). Judge the pH visually by thephenol red in the media then add your cell suspension.7) Pipette your wanted volume of the mixture into each well.8) Let 6-well plate sit at room temperature for 10-15minutes before placing in the incubator9) 1 hour later add your wanted volume of media on top of each Collagel Hydrogel (ACGH-002) (2-2.5 ml)10) Use within 1 week, change media every 2 days or as needed.