Bovine Collagen (4mg/ml)

Catalog Number TB02
Product Name Type I Bovine Collagen
Storage 2-8°C
Product Format Liquid
Volume 20ml
GENERAL INFORMATION
Collagen naturally plays an important role within the body, but it is being used increasingly within
certain areas of medicine as well. The breakdown of the body natural collagen can lead to a number of
health problems, but the protein can also be used in other contexts to help with the repair of the body.
Collagen comprises a family of genetically distinct molecules, all of which have a unique triple helix
configuration of three polypeptide subunits known as alpha-chains. Each chain contains around 1000
amino acids, and usually features an amino acid sequence consisting of glycine, proline and
hydroxyproline.
Our product unique characteristics
Shelf Life 2 years
Purity >95 % (SDS PAGE)
pH 3.0
Concentration 4mg /ml by Sircol Assay
Source Bovine Achilles Tendon
Buffer 0.2M Acetic Acid
Gel Information Gel in a few minutes after neutralizing the product
Great for coating plates, and T-flasks
Work very well when used in 3D tissue models

Caution:
The handling of any organism derived products has potential to be biologically hazardous. Proper
precautions must be taken to avoid exposure. Always wear proper protective equipment (Gloves, safety
glasses, etc.) when handling these materials. We recommend following the universal procedures for
handling products of any organism origin as the minimum precaution against contamination. All
products offered by Alphabioregen are for laboratory research purposes only. Any other use and results
of that use are the sole responsibility of the user and are not in any way the responsibility of
Alphabioregen.
Recommended Coating and Washing Technique
1-Add sufficient collagen solution to coat dishes, plates or inserts1-2 ml of solution is sufficient to cover
a 35 mm dish.
2-Incubate at room temperature in a biological safety cabinet partially covered for 1 hour.
3-Carefully aspirate remaining solution. Rinse with proper volume of serum – free media to remove
buffer solution.
4-Coated plates are best used immediately but can be air-dried and stored.
5-Coated plates that have not been cultured can be dried and stored at 2 -8 °C and are useful up to 1
week under sterile conditions
Well Area (cm²) Coating Volume (ml) Wash Volume(ml)
6 4.67 0.6 1
12 1.12 0.25 0.4
24 0.33 0.05 0.1
75 mm insert 44 5 8
96 0.143 0.025 0.05