Product Name: BGJb Medium Modified w/o L-GlutamineCatalog No: PG013Product Format: LiquidStored at 4°C w/o L-Glutamine
_____________________________________________________________________General Information: Medium BGJ was originally developed by Biggers, Gwatkin andJudah in the early 1960’s at the Wistar Institute. Subsequent studies resulted in amodification designated BGJb which has been used for supporting cultures of cartilaginousembryonic bone. An additional modification, developed by Sylvia Fitton-Jackson atStrangeways Laboratory in England, is further enriched over the prior formulae. Additionalamino acids and vitamins, and increased buffering capacity conferred by the phosphates inthe Fitton-Jackson modification, create conditions that permit calcification and growth ofcartilaginous embryonic bone.The medium is for research use only and not approved for human or animal use or forapplication in any in vitro diagnostic procedure. Storage of the medium andsupplements Please note that the basal medium has to be stored at 4°C up to 8°C butnever should be frozen. The supplement has to be stored at -20°C. Protect from light fora longer time period.Preparing and Stability:Thaw the supplement mixture at 37°C before adding to the basal medium. After thawingplease rinse the bottle and the tube with 70% ethanol as protection. Remove ethanolexcess before opening the cap of the tube. After adding the supplement mixture to thebasal medium slew carefully to get a homogenous medium. Please be careful not to touchthe tube or bottle interior threads with your fingers. Please avoid long term exposure tolight because some components are light sensitive. Also do not warm up the medium over37°C. All this protects your medium against contamination or degradation of essentialcomponents.