Product Name Anti-CDC6 Antibody (Monoclonal, DCS-180)Description Mouse IgG monoclonal antibody for CDC6, cell division cycle 6 homolog (S.cerevisiae) (CDC6) detection. Tested with WB in Human. No cross reactivitywith other proteins.Host MouseTarget Species HumanApplication WBRecommended Detection SystemsWe recommend Enhanced Chemiluminescent Kit with anti-Mouse IgG(EK1001) for Western blot.Immunogen Recombinant human Cdc6.Specificity No cross reactivity with other proteinsPack Size 100μg/vialCatalog # Ab1019Background for Cell division control protein 6 homologIn yeasts, Cdc6(Saccharomyces cerevisiae) and Cdc18(Schizosaccharomyces pombe) associate with theorigin recognition complex(ORC) proteins to render cells competent for DNA replication. Cdc6 isoverexpressed in human cancers, where it has a critical regulatory role in addition to DNA replication.Yan et al. (1998) showed that Cdc6 is expressed selectively in proliferating but not quiescent mammaliancells, both in culture and within tissues in intact animals. During the transition from a growth-arrested to aproliferative state, transcription of mammalian Cdc6 is regulated by E2F proteins, as revealed by afunctional analysis of the human Cdc6 promoter and by the ability of exogenously expressed E2Fproteins to stimulate the endogenous Cdc6 gene. They conclude that expression of human Cdc6 isregulated in response to mitogenic signals though transcriptional control mechanisms involving E2Fproteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells.
PropertiesClonality MonoclonalClone Number DCS-180Form LyophilizedContents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 aspreservative.*carrier free antibody available upon request.Reconstitution Add 1ml of PBS buffer will yield a concentration of 100ug/ml.Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can alsobe aliquoted and stored frozen at -20˚C for a longer time. Avoid repeatedfreezing and thawing.Purification AscitesApplication DetailsApplication Concentration* Tested Species Predicted Species Antigen RetrievalWesternblot1-2μg/ml Human –Protein Target Info (Source: Uniprot.org)Gene Name CDC6Protein Name Cell division control protein 6 homologGene Full Name cell division cycle 6 homolog(S. cerevisiae)Protein Function Involved in the initiation of DNA replication. Also participates in checkpointcontrols that ensure DNA replication is completed before mitosis is initiated.Sequence Similarities Belongs to the CDC6/cdc18 family.SubcellularLocalizationNucleus.