Anti-CDC6 Antibody (Monoclonal,DCS -180)

Description

Product Name Anti-CDC6 Antibody (Monoclonal, DCS-180)
Description Mouse IgG monoclonal antibody for CDC6, cell division cycle 6 homolog (S.
cerevisiae) (CDC6) detection. Tested with WB in Human. No cross reactivity
with other proteins.
Host Mouse
Target Species Human
Application WB
Recommended Detection Systems
We recommend Enhanced Chemiluminescent Kit with anti-Mouse IgG
(EK1001) for Western blot.
Immunogen Recombinant human Cdc6.
Specificity No cross reactivity with other proteins
Pack Size 100μg/vial
Catalog # Ab1019
Background for Cell division control protein 6 homolog
In yeasts, Cdc6(Saccharomyces cerevisiae) and Cdc18(Schizosaccharomyces pombe) associate with the
origin recognition complex(ORC) proteins to render cells competent for DNA replication. Cdc6 is
overexpressed in human cancers, where it has a critical regulatory role in addition to DNA replication.
Yan et al. (1998) showed that Cdc6 is expressed selectively in proliferating but not quiescent mammalian
cells, both in culture and within tissues in intact animals. During the transition from a growth-arrested to a
proliferative state, transcription of mammalian Cdc6 is regulated by E2F proteins, as revealed by a
functional analysis of the human Cdc6 promoter and by the ability of exogenously expressed E2F
proteins to stimulate the endogenous Cdc6 gene. They conclude that expression of human Cdc6 is
regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F
proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells.

Properties
Clonality Monoclonal
Clone Number DCS-180
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as
preservative.
*carrier free antibody available upon request.
Reconstitution Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also
be aliquoted and stored frozen at -20˚C for a longer time. Avoid repeated
freezing and thawing.
Purification Ascites
Application Details
Application Concentration* Tested Species Predicted Species Antigen Retrieval
Western
blot
1-2μg/ml Human –
Protein Target Info (Source: Uniprot.org)
Gene Name CDC6
Protein Name Cell division control protein 6 homolog
Gene Full Name cell division cycle 6 homolog(S. cerevisiae)
Protein Function Involved in the initiation of DNA replication. Also participates in checkpoint
controls that ensure DNA replication is completed before mitosis is initiated.
Sequence Similarities Belongs to the CDC6/cdc18 family.
Subcellular
Localization
Nucleus.

International:

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