Product Name Anti-CDC25C Antibody (Monoclonal, DCS-193)Description Mouse IgG monoclonal antibody for CDC25C, cell division cycle 25homolog C (S. pombe) (CDC25C) detection. Tested with WB in Human. Nocross reactivity with other proteins.Host MouseTarget Species HumanApplication WBRecommended Detection SystemsWe recommend Enhanced Chemiluminescent Kit with anti-Mouse IgG(EK1001) for Western blot.Immunogen Recombinant human Cdc25c.Specificity No cross reactivity with other proteinsPack Size 100μg/vialCatalog # Ab1018Background for M-phase inducer phosphatase 3Cell division sycle(CDC25) gene product is a protein-tyrosine phosphatase, activates a partially purifiedp34(cdc2)/cyclin B complex. The cdc25 protein also shares homology with a protein phosphatase withactivity against both tyrosine and serine(and thus probably threonine) phosphate residues. CDC25C isexpressed predominantly in G2 phase in healer cells. The human gene encodes a protein with apredicted molecular mass of 53,000 daltons whose C-terminal domain shares about 37% sequenceidentity with the fission yeast cdc25 mitotic inducer. CDC25C gene is mapped to 5q31.
PropertiesClonality MonoclonalClone Number DCS-193Form LyophilizedContents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 aspreservative.*carrier free antibody available upon request.Reconstitution Add 1ml of PBS buffer will yield a concentration of 100ug/ml.Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can alsobe aliquotted and stored frozen at -20˚C for a longer time.Avoid repeatedfreezing and thawing.Purification AscitesApplication DetailsApplication Concentration* Tested Species Predicted Species Antigen RetrievalWesternblot1-2μg/ml Human
Protein Target Info (Source: Uniprot.org)Gene Name CDC25CProtein Name M-phase inducer phosphatase 3Gene Full Name cell division cycle 25 homolog C(S. pombe)
Protein Function Functions as a dosage-dependent inducer in mitotic control. Tyrosineprotein phosphatase required for progression of the cell cycle. Whenphosphorylated, highly effective in activating G2 cells into prophase. Directlydephosphorylates CDK1 and activates its kinase activity.Sequence Similarities Belongs to the MPI phosphatase family.Subcellular Localization Nucleus.Uniprot ID Human(P30307), Mouse(P48967), Rat(D4ADT2)Alternative Names M-phase inducer phosphatase 3|CDC25C|Mphaseinducerphosphatase3|Mphase inducer phosphatase3|Mphaseinducerphosphatase3