Product Name Anti-Calbindin-D Antibody (Monoclonal, CB-955)Description Mouse IgG monoclonal antibody for Calbindin-D, calbindin 1,28kDa (CALB1) detection. Tested with WB, IHC-P inHuman;mouse;rat;bovine. No cross reactivity with other proteins.Host MouseTarget Species Bovine, Human, Mouse, RatApplication IHC-F, WBRecommendedDetectionSystemsWe recommends Enhanced Chemiluminescent Kit with anti-MouseIgG (EK1001) for Western blot, and HRP Conjugated anti-MouseIgG Super Vision Assay Kit (SV0001-1) for IHC(F). Contact us for more information.Immunogen Bovine kidney calbindin-DSpecificity No cross reactivity with other proteinsPack Size 100μg/vialCatalogNumberAb1007Background:The human calbindin D28k(CALB1) is a calcium-binding protein belonging to thetroponin C superfamily. It was originally described as a 27-kD protein induced byvitamin D in the duodenum of the chick. Parmentier et al.(1991) mapped the CALB1gene to 8q21.3-q22.1 by in situ hybridization.Product Properties:Clonality MonoclonalClone Number CB-955Form LyophilizedContents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mgNaN3 as preservative. *carrier free antibody available upon request.Reconstitution Add 1ml of 1XPBS buffer (catalog # 1XPBS-001) will yield aconcentration of 100ug/ml.Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. Itcan also be aliquoted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.Purification Ascites.
Application Details:Application Concentration*TestedSpeciesPredictedSpeciesAntigenRetrievalWestern blot 0.5-1μg/ml Human,bovine,mouse,rat–Immunohistochemistry(Paraffin-embedded Section)1-2μg/ml Human,bovine,mouse,rat– By HeatProtein Target:Gene Name CALB1Protein Name CalbindinGene Full Name calbindin 1, 28kDaProtein Function Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3′,5′-cyclic nucleotide phosphodiesterase.SequenceSimilaritiesBelongs to the calbindin family.Uniprot ID Human(P05937), Mouse(P12658), Rat(P07171)Alternative Names Calbindin-D|CalbindinD|CALB1