Product Name Anti-c-Myc Antibody (Monoclonal, 9E10)Description Mouse IgG monoclonal antibody for c-Myc, v-myc myelocytomatosis viraloncogene homolog (avian) (MYC) detection. Tested with WB, IHC-P, ICC inHuman. No cross reactivity with other proteins.Host MouseTarget Species HumanApplication IHC-P, ICC, WBRecommendedDetection SystemsWe recommend Enhanced Chemiluminescent Kit with anti-Mouse IgG(EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG SuperVision Assay Kit (SV0001-1) for IHC(P) and ICC.Immunogen Synthetic peptide corresponding to residues 408-439 of the human p62cMyc protein.Specificity No cross reactivity with other proteinsPack Size 100μg/vialCatalog # Ab1028PropertiesClonality MonoclonalClone Number 9E10Form LyophilizedContents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 aspreservative.*carrier free antibody available upon request.
Reconstitution Add 1ml of PBS buffer will yield a concentration of 100ug/ml.Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can alsobe aliquoted and stored frozen at -20˚C for a longer time. Avoid repeatedfreezing and thawing.Purification AscitesBackground for Myc proto-oncogene proteinC-Myc is an oncogene that functions both in the stimulation of cell proliferation and in apoptosis. c-Mycelicits its oncogenic activity by causing immortalization, and to a lesser extent the transformation of cells,in addition to several other mechanisms. The c-MYC proto-oncogene encodes a transcription factor thatis critical for cell growth and proliferation. It is one of the genes frequently altered in cancer cells in whichit exhibits constitutive activity. Downregulation of c-Myc is critical for 2-Methoxyestradiol(2ME2)-inducedoxidative stress and apoptosis in AML cells. And its up-regulation is important for promoting lymphocytecell division, and demonstrating that GFP-c-Myc expression is a marker of proliferating lymphocytes invivo.Application DetailsApplication Concentration* TestedSpeciesPredictedSpeciesAntigenRetrievalWestern blot 4μg/ml Human –Immunohistochemistry(Paraffinembedded Section)8μg/ml Human – By HeatImmunocytochemistry 1μg/ml Human –Price: $150Protein Target Info (Source: Uniprot.org)Gene Name MYCProtein Name Myc proto-oncogene proteinGene Full Name v-myc myelocytomatosis viral oncogene homolog(avian)
Protein Function Transcription factor that binds DNA in a non-specific manner, yet alsospecifically recognizes the core sequence 5′- CAC[GA]TG-3′. Activates thetranscription of growth-related genes.Sequence Similarities Contains 1 bHLH (basic helix-loop-helix) domain.SubcellularLocalizationNucleus, nucleoplasm.Uniprot ID Human(P01106), Mouse(P01108), Rat(P09416)Alternative Names MYC|Myc proto-oncogene protein|Mycproto-oncogeneprotein|Mycprotooncogene protein|Mycprotooncogeneprotein