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Product Name Anti-Bcl-2 antibody (monoclonal)Description Mouse IgG monoclonal antibody for Bcl-2, B-cell CLL/lymphoma 2(BCL2) detection. Tested with WB, IHC-P, IHC-F, ICC in Human.No cross reactivity with other proteins.Host MouseTarget Species HumanApplication WB,HC-P, IHC-F, ICC,RecommendedDetectionSystemsWe recommends Enhanced Chemiluminescent Kit with anti-MouseIgG (EK1001) for Western blot, and HRP Conjugated anti-MouseIgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) andICC.*Blocking peptide can be purchased. Contact us for moreinformationImmunogen Recombinant full-length BCL2Specificity No cross reactivity with other proteinsPack Size 100μg/vialCatalog Number Ab1004
Background:Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomaswhereas no BCL2 protein was detected in follicles affected by nonneoplastic processesor in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t(14;18)translocation expressed detectable levels of BCL2 protein, regardless of whether thebreakpoint was located in or at a distance from the BCL2 gene. Overexpression ofBCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.Product Properties:Clonality MonoclonalClone Number Bcl-2-100Form LyophilizedContents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mgNaN3 as preservative.*carrier free antibody available upon request.Reconstitution Add 1ml of 1XPBS buffer (catalog # 1XPBS-001) will yield aconcentration of 100ug/ml.Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. Itcan also be aliquoted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.Purification AscitesApplication Details:Application Concentration* TestedSpeciesPredictedSpeciesAntigenRetrievalWestern blot 1-2μg/ml Human –Immunohistochemistry(Paraffinembedded Section)0.4-1μg/ml Human – By HeatImmunohistochemistry(FrozenSection)0.4-1μg/ml Human –Immunocytochemistry 1μg/ml Human
Protein Target:Gene Name BCL2Protein Name Apoptosis regulator Bcl-2Gene Full Name B-cell CLL/lymphoma 2Protein Function Suppresses apoptosis in a variety of cell systems includingfactor-dependent lymphohematopoietic and neural cells.Regulates cell death by controlling the mitochondrial membranepermeability. Appears to function in a feedback loop systemwith caspases. Inhibits caspase activity either by preventing therelease of cytochrome c from the mitochondria and/or bybinding to the apoptosis-activating factor (APAF-1).Tissue Specificity Expressed in a variety of tissues.SequenceSimilaritiesBelongs to the Bcl-2 family.SubcellularLocalizationMitochondrion outer membrane.Uniprot ID Human(P10415), Mouse(P10417), Rat(P49950)Alternative Names Apoptosis regulator Bcl-2|BCL2|ApoptosisregulatorBcl2|Apoptosis regulator Bcl2|ApoptosisregulatorBcl2
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