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Product Name Anti-Actin Antibody (Monoclonal, AC-40)Description Mouse IgG monoclonal antibody for Actin detection. Tested with WB, IHC-F inHuman;mouse;rat;chicken. No cross reactivity with other proteinsHost MouseTarget Species Chicken, Human, Mouse, RatApplication IHC-F, WBRecommendedDetectionSystemsWe recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001)for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit(SV0001-1) for IHC(F).*Blocking peptide can be purchased. Contact us for more informationImmunogen Synthetic actin C-terminal peptideSer-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-CysPhe,attached to a Multiple Antigen Peptide(MAP) backbone.Specificity No cross reactivity with other proteinsPack Size 100μg/vialCatalog Number Ab1000
Background for Actin:Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structuresin the cell types. It varies in amount, being related to the type of differentiation and to the functional stateof cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a uniqueNH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gammaactin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibodyhas been raised using a synthetic peptide corresponding to the C-terminal 11 residues.Product Properties:Clonality MonoclonalClone Number AC-40Form LyophilizedContents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 aspreservative. *carrier free antibody available upon request.Reconstitution Add 1ml of 1XPBS buffer (catalog # 1XPBS-001) will yield a concentration of100ug/ml.Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also bealiquoted and stored frozen at -20˚C for a longer time. Avoid repeated freezing andthawing.Purification AscitesApplication Details:Application Concentration* TestedSpeciesPredictedSpeciesAntigenRetrievalWestern blot 2μg/ml Human,chicken,mouse, rat–Immunohistochemistry(FrozenSection4μg/ml Human,chicken,mouse, rat
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