Anti – Actin Antibody (Monoclonal, AC – 40)

Description

Product Name Anti-Actin Antibody (Monoclonal, AC-40)
Description Mouse IgG monoclonal antibody for Actin detection. Tested with WB, IHC-F in
Human;mouse;rat;chicken. No cross reactivity with other proteins
Host Mouse
Target Species Chicken, Human, Mouse, Rat
Application IHC-F, WB
Recommended
Detection
Systems
We recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001)
for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit
(SV0001-1) for IHC(F).
*Blocking peptide can be purchased. Contact us for more information
Immunogen Synthetic actin C-terminal peptideSer-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-CysPhe,attached to a Multiple Antigen Peptide(MAP) backbone.
Specificity No cross reactivity with other proteins
Pack Size 100μg/vial
Catalog Number Ab1000

Background for Actin:
Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures
in the cell types. It varies in amount, being related to the type of differentiation and to the functional state
of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique
NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gammaactin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody
has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.
Product Properties:
Clonality Monoclonal
Clone Number AC-40
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as
preservative. *carrier free antibody available upon request.
Reconstitution Add 1ml of 1XPBS buffer (catalog # 1XPBS-001) will yield a concentration of
100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be
aliquoted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and
thawing.
Purification Ascites
Application Details:
Application Concentration* Tested
Species
Predicted
Species
Antigen
Retrieval
Western blot 2μg/ml Human,
chicken,
mouse, rat
–
Immunohistochemistry(Frozen
Section
4μg/ml Human,
chicken,
mouse, rat

International:

May vary, we will contact you!