Catalog Number :PG-003Product Name:AlphaBioGelStorage: -20°CProduct Format: FrozenGENERAL INFORMATIONIn classic tissue engineering and regenerative medicine research combines cells and scaffolds, inducedto growth with biochemical and mechanical stimuli, in a laboratory setting for organ repair andreplacement, AlphaBioGel (PG-003) composed extracellular matrix (ECM) fibrous protein moleculessecreted by cells. AlphaBioGel (PG-003) provides structural and biochemical support to the surroundingcells in 3D tissue models. AlphaBioGel (PG-003) increase cell adhesion, cell-to-cell communication anddifferentiation. AlphaBioGel (PG-003) fibrous proteins fill the interstitial space and act as a compressionbuffer against the stress placed on the 3D tissue model, while helping cells build strong basementmembranes. In terms of wound healing, AlphaBiogel (PG-003) has two main purposes. First, it preventsthe immune system from triggering from the injury and responding with inflammation and scar tissue.Next, it facilitates the surrounding cells to repair the tissue instead of forming scar tissue. AlphaBioGel(PG-003) can also be used to support 3D cell culture in vitro for modeling tumor development.PrecautionsEverything should be kept cold to avoid the AlphaBioGel (PG-003) from solidifying. Avoid airbubbles. This protocol is based on 6- well plate usage.MethodsThaw your AlphaBioGel (PG-003) sample bottle at room temp or in a 37ºC water bath, invertproduct while thawing. When you see a small amount of ice left in your sample, transfer thebottle into an ice bath. It is important to keep AlphaBioGel (PG-003) on ice since it willsolidify at temperatures above 8ºC.