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Product Name AlphaBioCoatCatalog Number AC001Product Format LiquidStorage 2-8° C
*Caution: The handling of human derived products has potential to be biologically. All Cell strains tested negative for HIV, HBV, and HCV DNAin diagnostic tests. Proper precautions must be taken to avoid exposure. Always wear proper protective equipment (Gloves, safety glasses, etc.)when handling these materials. We recommend following the universal procedures for handling products of human origin as the minimumprecaution against contamination.GENERAL INFORMATIONCell culture can be a very difficult tool to work with in the laboratory. Universal BioProducts often receives callsfrom anxious customers who are experiencing growth or attachment problems with their cell lines.Growth and attachment problems with cell lines are caused by:1. Poor surface treatment2. Culture media3. Sera4. High conductivity of the vesselsUniversal BioProducts has spent many years helping customers dealing with these problems. Making your cellbehave is not that hard. It’s all about positive reinforcement. The best product that will do extracellularmatrix (ECM) that provides structural and biochemical support to the surrounding cells. It facilitates cellgrowth and attachment.Our product unique characteristics:Product Name AlphaBioCoatCatalog Number AC001Product Format LiquidStorage 2-8° C2 years shelf lifeGreat for Coating plates, and T-flasksWork very well when used in 3D tissue modelsFrom gel in a few minutes after neutralizing the productPurity > 98% (SDS PAGE)
Product uses:A) Coating a T-25 flask: add 2ml of AlphaBioCoat solution into one T-25 flask and make sure wholesurface is covered with the coating solution.B) Place Flask in a 37degrees Incubator for Thirty minutes, dispose AlphaBioCoat solution byaspiration and the flask is ready to be used (no need for overnight incubation when coated withAlphaBioCoat solution)C) Gently rinse the cells in T-25 flask with 5ml1XPBS (Code 300) room temperature (RT) twice.Make sure the plate is at a neutral pH before seeding cells into T-25 flask.D) Remove Cells from the liquid Nitrogen, place in a water to thaw cells. (Note: Don’t spin the cellsduring the subculture process.E) Suspend the cells with 5ml of Media. Transfer Cells suspension directly into 1X pre-coated T-25Flask.F) Place Cells in a 37 Degrees Incubator.G) Cell Culture Protocol (Proliferating).H) Culture Media is changed every 2 days
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