Product Name AlphabioDetach Cell Detachment SolutionCatalog number ADF001Storage 2-8°CSize 50mlCaution: Proper precautions must be taken to avoid exposure. Always wear proper protective equipment (Gloves, safety glasses, etc.) whenhandling these materials. We recommend following the universal procedures for handling products of human origin as the minimum precautionagainst contamination.DescriptionThe AlphabioDetach Cell Detachment Solution is an effective alternative to Trypsin/EDTA for in vitrocultured cells, containing proteolytic and collagenolytic enzymes. The AlphabioDetach is a replacementfor TRYPSIN/EDTA solution and useful for the detachment of cells from standard tissue culture plates orT-flasks. AlphabioDetach contains Protease and collagenase activities in an isotonic, phosphate buffersolution with EDTA. It works successfully well on detaching primary cells and cell lines. In summary,AlphabioDetach Cell Detachment Solution offers the following benefits:1- Maximum cells viability2- No need to wash detached cells3- Gentle and rapid detachment4- Effective on a wide range of cells5- No mammalian or bacterial by-products6- Stable between 2°C and 8°C for 2 monthsThis product is shipped with an ice pack. It may thaw during shipment. Upon receipt, store at -20°C. Itcan be used for 2D and 3D cultured cells.
Usage:1) Thaw the AlphabioDetach Cell Detachment Solution at room temperature.2) After thawing, AlphabioDetach can be stored between 2°C and 8°C for up two months. For longterm storage, keep AlphabioDetach at -20°C.3) Remove cell culture media4) Rinse culture plates or T-Flasks with IXPBS-001.5) Dispose of 1XPBS by aspiration6) Add an adequate volume of AlphabioDetach Cell Detachment Solution to cover cells. Useminimum of 1.0ml per 75-cm² surface area and rock container back and forth to cover surfacearea with AlphabioDetach.7) Allow cells to detach at room temperature or 37°C Incubator for 5-10 minutes, or until the cellsare detached.8) Monitor cell detachment. Strike the flask against palm of hand to detach cells.9) After cells have detached, add growth media at a volume equal to the original Detachmentsolution.10) Gently pipette the cells suspension up and down 5 times with the culture plates or T-Flasks inother to flush out the remaining cells from the culture plates or T-Flasks.11) Count cells, passage, or process as needed.12) Cells are ready to be used for reseeding or experimental analysis.