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Catalog Number AGEM-001Product Name Alpha-Glia Expansion MediumStorage 4°CSize 500mL
*Caution: The handling of human derived products has potential to be biologically. All Cell strains tested negative for HIV, HBV, and HCVDNA in diagnostic tests. Proper precautions must be taken to avoid exposure. Always wear proper protective equipment (Gloves, safety glasses,etc.) when handling these materials. We recommend following the universal procedures for handling products of human origin as the minimumprecaution against contamination.GENERAL INFORMATION:Microglial cells are the dominant antigen presenting cells in the central nervous system. Underresting conditions, the expression of the molecular complex for presenting antigen, the majorhistocompatibility complex II (MHCII) and co-stimulatory molecules such as CD80, CD86 andCD40 is below detection. Upon injury the molecules are highly upregulated and the expressionof this complex is essential for interacting with T lymphocytes. This upregulation has beendescribed in a number of pathologies and is well studied in Multiple Sclerosis. Microglial cellsphagocytose myelin, degrade it and present peptides of the myelin proteins as antigens. Byreleasing cytokines such as CCl2 microglial cells are important for recruiting leucocytes into theCNS. Microglia interact with infiltrating T lymphocytes and, thus, mediate the immune responsein the brain. They have the capacity to stimulate proliferation of both TH1- and TH2-CD4positive T cells.Alpha-Glia Expansion Medium is a complete medium designed to promote optimal growth ofnormal human brain-derived microglia in vitro. It is a sterile, liquid medium which contains:• Essential and non-essential amino acids• Vitamins• Organic and inorganic compounds• Hormones• Growth factors• Trace minerals• Fetal bovine serum (5%).The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator withan atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively)to provide a defined and optimally balanced nutritional environment that selectively promotesgrowth of normal human microglia in vitro.Alpha-Glia Expansion Medium consists of 445 ml of basal medium, 25 ml of fetal bovine serum25 ml of microglia growth supplement and 5 ml of penicillin/streptomycin solution.
Shipping Condition: Ambient temperature (Blue ice, seasonally)Storage condition: Alpha-Glia Expansion Medium is stored at 4°C in the dark. A change incolor or appearance of precipitate may indicate deterioration.Shelf Life: Alpha-Glia Expansion Medium is stable for at least 2 months from the date ofreceiving under proper storage conditionUse of Alpha-Glia Expansion Medium for culturing human Microglial cells:1)-Take the amount of Alpha-Glia Expansion Medium needed and warm to 37°C in a waterbath or incubator2)-Avoid frequent temperature change to the entire bottle of medium.3)-Then Alpha-Glia Expansion Medium are stable for at least 1 month stored at 4°C.4)-We recommend changing medium every 2 days for regular cell culture.
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