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Product Name 3D Human Blood BrainBarrierCatalog Number EP010Product Format 6 , 12, and 24 wellStorage Temperature -80℃.Shipping Temperature -80℃.CNS pharmaceutics market: Only a few central nervous system (CNS) disorders, such asdepression, epilepsy, chronic pain, and affective disorders, respond to clinical treatmentsby the 2% of small-molecule drugs that we have; on the other hand, many more seriousCNS disorders cannot be effectively treated by these small therapeutic molecules includingAlzheimer disease, Huntington disease, stroke, brain cancer, brain and spinal cord injury,HIV infection of the brain, therefore we want to help change that by creating our 3DHumanBBB model. The blood brain barrier (BBB) specifically regulates molecular and cellularflux between the blood and the nervous tissue. We develop and characterize a highlyreproducible Human in vitro model of the BBB using co-cultures of primary Human brainendothelial cells (HBEC), Human brain pericytes, and Human brain astrocytes to studyreceptors involved in transcytosis across the endothelial cell monolayer. Many drugsdeveloped to treat Central Nervous System (CNS) disorders are unable to reach the brainparenchyma in therapeutically relevant concentrations. The BBB protects brain nervoustissue from the fluctuation of plasma composition, from pathogenic agents, and maintainshomeostasis of the brain parenchyma by restricting non-specific flux of ions, peptides,proteins and even cells into and out the brain.Astrocytes and brain pericytes help to develop and maintain specific BBB characteristics inbrain capillary endothelial cells. Co-culture of the three cell types in our 3D Human BBBmodel led to the enhancement of barrier properties; an increase in expressions of tightjunction proteins of occludin, claudin-5 and ZO-1 and continuous localizations of ZO-1 andclaudin-5.Our model mimic transport properties of the BBB due to the formation of tight junctions,higher expression of specific carriers, or great cell viability. We developed a 3D in vitromodel of the BBB by culturing brain endothelial cells with pericytes and astrocytes layered in an insert. This model improves endothelial cell polarization and enhance theformation of tight junctions, provide better endothelial cell-to-cell contact that isimportant for barrier development, and prevent the dilution of secretedneurotrophic factors, and these conditions collectively led to the development of an invitro model that can truly mimic the BBB.Advantages:1) Cells used in the 3D model are all human cells; results obtained are more relevant tohuman situations rather than those data from animal models, i.e. CAM et al.2) The whole process can be monitored (from cell inoculation to the end ofexperiment), therefore, more crucial information can be acquired at multiple timepoints from a single experiment.3) No need to perform post-experimental staining for endothelial markers, this isparticularly important, if those markers are changed in experimental conditionsinvolved in the studies.The 3D Human BBB Model contains all the materials necessary to perform multipleangiogenesis assays in 6, 12, or 24 well formats.The 3D Human BBB model can be use, but not limited to:• Drug BBB permeability assay• Research on BBB physiology• Cell-cell interactions• Transport pathway modulations• Research on BBB toxicology• Brain endothelial toxicity assays• Research on BBB pathology• Disease modeling Microvessels• Transport and permeability studies from ions to macromolecules: effect ofphysiological or pathogenetic factors• Paracellular barrier and cell polarity studies: TJ protein expression, distribution,polarized distribution of transport proteins, receptors, enzymes etc• Studies on endo- and transcytosis, receptor-ligand interactions • Drug transport, drug effect on permeability, localization of receptors, polarity ofdrug responses• Co-culture studies: cell-cell and cell-matrix interactions• Microbial pathogenesis: virus, bacteria, parasite attachment, invasion andpenetration• Compounds screening neuroimmune targets
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