Anti – CDC25C Antibody (Monoclonal, DCS – 193)

Description

Product Name Anti-CDC25C Antibody (Monoclonal, DCS-193)
Description Mouse IgG monoclonal antibody for CDC25C, cell division cycle 25
homolog C (S. pombe) (CDC25C) detection. Tested with WB in Human. No
cross reactivity with other proteins.
Host Mouse
Target Species Human
Application WB
Recommended Detection Systems
We recommend Enhanced Chemiluminescent Kit with anti-Mouse IgG
(EK1001) for Western blot.
Immunogen Recombinant human Cdc25c.
Specificity No cross reactivity with other proteins
Pack Size 100μg/vial
Catalog # Ab1018
Background for M-phase inducer phosphatase 3
Cell division sycle(CDC25) gene product is a protein-tyrosine phosphatase, activates a partially purified
p34(cdc2)/cyclin B complex. The cdc25 protein also shares homology with a protein phosphatase with
activity against both tyrosine and serine(and thus probably threonine) phosphate residues. CDC25C is
expressed predominantly in G2 phase in healer cells. The human gene encodes a protein with a
predicted molecular mass of 53,000 daltons whose C-terminal domain shares about 37% sequence
identity with the fission yeast cdc25 mitotic inducer. CDC25C gene is mapped to 5q31.

Properties
Clonality  Monoclonal
Clone Number  DCS-193
Form  Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as
preservative.
*carrier free antibody available upon request.
Reconstitution Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also
be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated
freezing and thawing.
Purification Ascites
Application Details
Application Concentration* Tested Species Predicted Species Antigen Retrieval
Western
blot
1-2μg/ml Human

Protein Target Info (Source: Uniprot.org)
Gene Name CDC25C
Protein Name M-phase inducer phosphatase 3
Gene Full Name cell division cycle 25 homolog C(S. pombe)

Protein Function Functions as a dosage-dependent inducer in mitotic control. Tyrosine
protein phosphatase required for progression of the cell cycle. When
phosphorylated, highly effective in activating G2 cells into prophase. Directly
dephosphorylates CDK1 and activates its kinase activity.
Sequence Similarities Belongs to the MPI phosphatase family.
Subcellular Localization Nucleus.
Uniprot ID Human(P30307), Mouse(P48967), Rat(D4ADT2)
Alternative Names M-phase inducer phosphatase 3|CDC25C|Mphaseinducerphosphatase3|Mphase inducer phosphatase
3|Mphaseinducerphosphatase3

International:

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